Journal: Biology

Article Title: Digital PCR: What Relevance to Plant Studies?

doi: 10.3390/biology9120433

Figure Lengend Snippet: The main applications of digital PCR (dPCR) developed so far in the plant science sector are reported and classified into six main categories. The percentage of each category was calculated on the basis of number of recently published peer-reviewed studies. The literature screening was carried out using dPCR and its variants as main key words for an analysis of the recently published literature.

Article Snippet: In 2011, droplet digital PCR (ddPCR) was proposed by Quantalife Co, LT, as a cheaper approach in comparison to the microfluidic one.

Techniques: Digital PCR

Journal: Biology

Article Title: Digital PCR: What Relevance to Plant Studies?

doi: 10.3390/biology9120433

Figure Lengend Snippet: The table shows recently developed dPCR assays aimed at identifying and quantifying plant pathogens.

Article Snippet: In 2011, droplet digital PCR (ddPCR) was proposed by Quantalife Co, LT, as a cheaper approach in comparison to the microfluidic one.

Techniques: Virus, Infection

Journal: Biology

Article Title: Digital PCR: What Relevance to Plant Studies?

doi: 10.3390/biology9120433

Figure Lengend Snippet: Main advantages and disadvantages of dPCR technology.

Article Snippet: In 2011, droplet digital PCR (ddPCR) was proposed by Quantalife Co, LT, as a cheaper approach in comparison to the microfluidic one.

Techniques: Quantitative Proteomics, Low Copy Number, Variant Assay

Journal: Molecular psychiatry

Article Title: Functional implications of a psychiatric risk variant within CACNA1C in induced human neurons

doi: 10.1038/mp.2014.143

Figure Lengend Snippet: a . Construction of lentivirus vectors for generating iNs from human fibroblasts. b–e. Human iNs have neuronal morphology and express several neuronal markers, Tuj1 ( b ), MAP2 ( c ), NeuN ( d ) and hSynapsin promoter driving DsRed ( e ) three weeks following induction. f. Human iNs co-cultured with human astrocytes express a presynaptic marker, VGLUT1. g. RT-PCR analyses reveal human iNs express neuronal markers including FOXP2 , TBR1 , GRIN1(NR1) , GABRA6 , GABRB2 , NTRK2 (TrkB), and SLC17A7 (VGLUT1) , but not an inhibitory neuronal marker, SLC32A1(VGAT) . h. Representative traces showing action potentials with injected currents. Scale bars, 50 μm.

Article Snippet: These fibroblasts were genotyped by droplet digital PCR at the Broad Institute.

Techniques: Cell Culture, Marker, Reverse Transcription Polymerase Chain Reaction, Injection

Journal: Clinical chemistry

Article Title: Prospective Molecular Profiling of Circulating Tumor Cells from Patients with Melanoma Receiving Combinatorial Immunotherapy

doi: 10.1373/clinchem.2019.307140

Figure Lengend Snippet: Characteristics of AJCC patients with stage III/IV metastatic melanoma.

Article Snippet: WGA of melanoma CTC-derived DNA (CTC-DNA) isolated in vitro and from patients with melanoma were subjected to the RainDrop Digital Droplet PCR BRAF V600E Assay (RainDance™ Technologies) and performed according to the manufacturer’s protocol.

Techniques:

Journal: Clinical chemistry

Article Title: Prospective Molecular Profiling of Circulating Tumor Cells from Patients with Melanoma Receiving Combinatorial Immunotherapy

doi: 10.1373/clinchem.2019.307140

Figure Lengend Snippet: (A), patient 1 with left calf in-transit melanoma. Patient experienced PD with metastasis to upper left groin on pembrolizumab despite partial response at left calf lesions. (B), patient 2 with multiple pelvic lymph nodes and brain metastasis. Brain lesions displayed partial response, but pelvic lymph node disease progressed on bevacizumab treatment. (C), patient 3 with metastases to right groin lymph nodes and gluteal region. Melanoma progressed to new multifocal region in left buttock area while on nivolumab and dabrafenib/trametinib. BRAF V600E mutation in CTCs were detected upon PD despite receiving dabrafenib/trametinib. (D), patient 4 with metastatic melanoma in chest/back/ buttock. While on nivolumab/dabrafenib/trametinib treatment, PD occurred (i.e. intracranial progression). (E), patient 5 with multiple metastases (lung, liver, and spleen). Patient experienced progression to the brain and in the liver after 8 weeks despite partial response to nivolumamb and dabrafenib/trametinib by clinical standards. (F), patient 6 following complete right groin lymph node dissection. Patient recurred with multiple dermal metastases after a 10 month disease–free period. Pink panels denote PD according to Response Evaluation Criteria in Solid Tumors 1.1 criteria. Abbreviations: Wks, weeks; *Ipi, ipilimumab; NED, no evidence of disease; red dashed line, LDH activities; blood-CTCs, detectable mRNA biomarkers (≥2 biomarkers) in isolated CTCs; BRAF mutation, detectable BRAF mutation in isolated CTCs.

Article Snippet: WGA of melanoma CTC-derived DNA (CTC-DNA) isolated in vitro and from patients with melanoma were subjected to the RainDrop Digital Droplet PCR BRAF V600E Assay (RainDance™ Technologies) and performed according to the manufacturer’s protocol.

Techniques: Mutagenesis, Dissection, Isolation